Effective Fractions and Micro Biota of Fermented Juice of Epiphytic Lactic Acid Bacteria (FJLB) of Rice Plant (Oryza sativa L.)

Abstract

Fermented juice of epiphytic lactic acid bacteria (FJLB) produced from whole rice crop was fractionated into bacterial cells and cell-free supernatant, and additive effects of the fractions on the fermentation quality of silage were investigated. Micro biota of FJLB and silages with and without FJLB were also identified. Addition of the bacterial cells greatly improved silage quality, by increasing lactic acid and decreasing butyric acid. The cell-free supernatant, on the contrary, did not improve fermentation quality of silage. The micro biota in the FJLB changed distinctly with incubation time, with the major species being Lactococcus lactis subsp. lactis, Lactobacillus plantarum and Lactobacillus fermentum after 2 days, and L. fermentum and L. plantarum after 5 days of incubation. For the silages, the major species were Enterococcus mundtii and L. lactis subsp. lactis in the control (no addition of fractions), and L. plantarum with the FJLB.