Abstract
We assessed an effect of inoculation with glycerol and Lactobacillus coryniformis strain 394 which ferments glycerol to an antimicrobial component 3-hydroxypropionaldehyde (3-HPA) in laboratory-scale ensiling of whole-crop maize. Although 3-HPA was produced in the silage, it disappeared after 3 days of storage perhaps due to chemical reaction of 3-HPA and some other component in the silage. Nevertheless, it was observed after opening the silos (after 60 days of storage) inoculation with L. coryniformis 394 and glycerol proved to retard aerobic spoilage of the silage. In another experiment of cultivating spoilage-causing yeast (Pichia sp. Y1) in extract of the material maize which was previously supplemented with 3-HPA and incubated prior to inoculation with Pichia sp. Y1, 3-HPA disappeared after the incubation. In this extract, the growth of Pichia sp. Y1 was also retarded comparing to its growth in extract of the maize supplemented with no 3-HPA. This result suggests that the chemical reaction of 3-HPA and components in maize might produce some other antimicrobial component.
