Abstract
Biomimetic nano-apatite collagen composite is a promising biomaterial for bone tissue engineering. However, the low mechanical property and poor shaping property limited its application. We had prepared a three-dimensional scaffold material using a mineralized collagen and a degradable polymer, named as nHAC/PLA, which satisfied both the high biocompatibility and enough mechanical properties. The co-culture experimental results with human neutrophils demonstrate that this biomaterial has almost no initial inflammations. The osteoblasts were separated from the neonatal rat calvaria. By the static cell culture, we developed the three-dimensional osteoblasts/composite bone-like construct in vitro. It was shown by CLSM and OM observation that the cells could spread and proliferate throughout the inner-pores of the scaffold material.
