Adhesion of L929 cell on the HMDSO surfaces with different wettability

Abstract

Thin films of HMDSO (hexamethyldisiloxane) were plasma-polymerized on the 15mm round Thermanox coverslips, followed by low energy O₂ plasma treatment with different time duration. The surface wettability was characterized by contact angle measurement with distilled water. By controlling the O₂ treatment time, 6 kinds of discs with surface contact angles respectively 0, 20, 40, 60, 80 and 110 degrees were produced. The wettability of the surfaces was kept stable when stored in distilled water for 24 hours. The fibroblast cell line L929 was used in the following experiment. The discs were put into 24 well culture dish and cells were seeded with a density of 20000 per well. In 6, 12 and 24 hours the adhesion behavior of the cells on surface with different wettability was investigated. The results showed that cells seeded on hydrophilic surfaces attached more quick and larger quantity than hydrophobic ones.