Effects of Ni ions on cell viability and NO Production of murine peritoneal exudates cells (PEC) collected by lavage from thioglycolate injected mice

Abstract

To clarify and overcome the inflammatory/immunological phenomenon by Ni ions, we examined the effect of cell viability and NO production of murine peritoneal exudates cells (PEC) collected by lavage from thioglycolate injected mice exposed to LPS and Ni ions. The cell viability declined with incrementing Ni ion concentrations. NO production was maximized at Ni ion concentration of 50umol/L, followed by gradual decline and disappearance. Cell damage by Ni ions might be attributed to a complicated cell signaling system.