LD50高濃度Ni2+イオンがマクロファージ様細胞RAW264の遺伝子発現と活性酸素除去酵素（SOD）量に及ぼす影響評価

平 雅之; 佐々木 かおり; 斎藤 設雄; 荒木 吉馬

doi:10.14832/gsjsdmd.2005s.0.26.0

45th General Session of the Japanese Society for Dental Materials and Devices

Session ID : A-26

DOI https://doi.org/10.14832/gsjsdmd.2005s.0.26.0

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Host: The Japanese Society for Dental Materials and Devices

Gene expression and super-oxide dismutase (SOD) activities of macrophage-like RAW264 cells exposed to LD₅₀ high-dose Ni²⁺ ions

*Masayuki Taira, Kaori Sasaki, Setsuo Saitoh, Yoshima Araki

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Abstract

We evaluated m-RNA gene-expression of RAW264 cells exposed to high dose of Ni²⁺ ion (500micro-mol/L) by DNA microarray. After 1 day culture, 111 genes were significantly expressed. Characteristically, SOD1, metallothionein 2 and 70kDa heat shock protein genes were up-regulated, while cyclin A1 gene was down-regulated. SOD activity was also dose-dependently increased by Ni²⁺ ion.

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