Abstract
To develop a new biodegradable scaffold biomaterial reinforced with a frame, CO3Ap, which has crystallinity and a chemical composition similar to bone, was synthesized at pH 7.4 and 60 C. Then, the CO3Ap was mixed with a neutralized collagen gel and the CO3Ap-collagen mixtures were lyophilized into sponges in a HAp frame ring with 0.5 mm pores. Mouse osteoblast MC3T3-E1 cells were cultured on the CO3Ap-collagen sponge. Furthermore, these sponge-frame complexes were implanted beneath the periosteum cranii of rats. A sectional view of hematoxylin-eosin staining confirmed that osteoblast cells had well invaded the CO3Ap-collagen sponge. At the animal experiment, newly created bone was observed toward the inner core of the complex from the surface of the rat periosteum cranii after 8 wks. When these sponge-frame complexes with rh-BMP2 were implanted beneath the periosteum cranii of rats, sufficient new bone was created after 4 wks implantation.
