Biological response of Octacalcium Phosphate Combined with collagen implanted in subperiosteal region

Abstract

Previous studies showed that octacalcium phosphate (OCP) combined with collagen (OCP/Col) enhances bone regeneration in bone defect. In the present study, OCP/Col disc (1 mm or 3 mm in thickness) were implanted into the periosteal region in rat crania to investigate the bone regenerative properties of OCP/Col. The maximum thickness of the bone with implant was measured parallel to the calvaria. The histological sections were cut and activities of tartrate resistant acid phosphatase (TRAP) were stained to examine phagocytotic multinucleated giant cells. The effect of implantation on crystalline phase of OCP in the implant was examined by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The present study suggests that new bone formation on OCP/Col and biodegradation of OCP/Col are depending on the thickness of OCP/Col.