Abstract
Accumulation of endogenous AGEs (advanced glycation endproducts) in the body due to glycative stress is one of the factors contributing to aging and the progression of age-related degenerative diseases. Methods to control glycative stress include controlling postprandial hyperglycemia, inhibiting AGE formation, and degrading and excreting AGEs. Since there are multiple pathways for glycation in vivo, in order to prevent AGE accumulation, it is necessary to inhibit the multifactorial pathways of the glycation. From these perspectives, a combination of materials is important for inhibition of AGE formation. Previous studies have shown that amino acids inhibit the formation of fluorescent AGEs (F-AGEs) and pentosidine. In this study, we prepared mixed solutions containing either two or six amino acids, selected seven amino acids known to have inhibitory effects on AGE formation, and examined their effectiveness based on their varying inhibitory effects on F-AGE and pentosidine formation. A model in which glucose was reacted with human serum albumin or type 1 collagen was used for the experiment. The results showed that the amino acid mixture had a higher inhibition rate of pentosidine formation than F-AGE formation. Ultimately, we identified to the combination with highest rate of inhibition for both F-AGE and pentosidine formation. This amino acid mixture may inhibit pentosidine formation, thereby reducing its cross-linking potentially preventing protein denaturation and dysfunction.
