Antiglycative effect of ferulic acid.

Abstract

Glycative stress is one of the factors that cause aging and accelerates development of various diseases, including diabetes. Inhibition of glycative stress is referred to as anti-glycation. Anti-glycation includes suppression of postprandial hyperglycemia, suppression of glycative reaction, and decomposition and excretion of advanced glycation endproducts (AGEs). Inhibitory effects of AGE formation are recognized in various food materials such as herb teas, vegetables and fruits. It is reported that these food materials include many phenolic compounds that inhibit the formation of AGEs. Ferulic acid (FA) is a kind of phenolic compounds included in grains such as rice, wheat, fruits, vegetables, and other food materials. Bran (grain), in particular, includes a lot. FA is well known to be effective in inhibiting development of complications of diabetes or other neurotic diseases because of its anti-glycative / anti-inflammatory action. In our body, many kinds of AGEs are produced through many paths due to glycative stress, while there are many kinds of proteins that are glycated. On the other hand, reducing the function of proteins due to glycation is associated with the cross-linking formation induced by proteins changing into AGEs. The objective of this study was to evaluate the anti-diastatic effect of FA refined from rice bran. In this study, inhibitory effects of protein glycation reaction models such as human serum albumins (HSA), collagen and elastin, protein cross-linking formation by AGEs, and activity enhancement for oxidized protein hydrolase (OPH) were validated. As a result, it was clarified that FA inhibits the formation of fluorescent AGEs (F-AGEs) in all glycation reaction models. FA inhibited the formation of F-AGEs, pentosidine, CML, 3DG, GO, and MGO in the glycation reaction models of HSA-gulcose. In addition, FA also inhibited the formation of dimmer and trimer caused by the glycation of lysozyme. FA increased the OPH activation by a factor of 1.6. There is a possibility that anti-diastatication is associated with FA's usefulness for a variety of diseases.