Abstract
The use of high hydrostatic pressure has been a useful tool for studying physicochemical properties of proteins. Since proteins change their volume during reactions, pressure perturbs the equilibria and the relative populations, between the multiple conformers. We have been developing a high-pressure cell for HP-SAXS that has a small sample volume (ca. 0.05mL) and sufficiently high reproducibility, to collect solution scattering data from large protein complexes. We report the present status of the development and its application for large protein complex.
