Preparation of Molecular Imprinting Polymers and Its Application to Regioselective Hydrolysis of D-Galactose Derivatives

Abstract

D-Galactose-containing glycoconjugates play an important role in recognition of cell and immunity. Influenza virus recognizes sialyl(2-3)galactose or sialyl(2-6)galactose residues, such as hemagglutinin on the viral envelope. However, the synthesis of sialyl galactose derivatives needs many protection and deprotection steps, therefore the regioselective deprotection of D-galactose derivatives is required. Molecular Imprinting Polymer (MIP) attracts attention as molecular recognizing artifical polymer corresponding to antibodies and enzymes. Initially, we synthesized templates for the preparation of MIP in order to regioselectively hydrolyze the acetyl groups of 3- or 6- position of D-galactose derivatives, respectively. And then, we synthesized MIP by polymerization reaction. We report the regioselective hydrolysis of the acetyl groups of D-galactose derivatives catalyzed by MIP by monitoring with HPLC.

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