STUDIES ON CULTURE OF THE LEPROSY BACILLUS (Part 3)

Abstract

A new ground medium was consisted of Kirchner's medium added with serum and extract of murine leproma. Various amino acids, vitamins, phthiocol, 6-amino capron acid (ACA) were mixed to the above ground medium. Besides the oridinary method, double layers method (Fig 5), double tubes method (Fig 6) and intraperitoneal culture in white rats were applied. The raw or X-ray treated murine bacilli were cultured without any multiplication.
The human bacillus was inoculated upon the double fluid medium added with sphingomyelin and the result was negative.
These results of the various cultures lead us to have disapproval against the possibility of in vitro culture of the human and murine bacilli supported by some workers.