Multiplication of Mycobacterium lepraemurium in cell-free liquid medium
5. Establishment of NC-5 medium
1973 Volume 42 Issue 4 Pages 205-209
Details
1973 Volume 42 Issue 4 Pages 205-209
In the present paper, it was described that newly improved cell-free culture me-dium, referred to as NC-5, permitted more abundant multiplication of M. lepraemurium than that of NC-3 medium which was previously reported. The NG-5 medium was established by the method in which 1-cysteine HC1 was added to NC-3 medium. Opti-mal concentration of 1-cysteine HC1 to be added was 0.03%. When three strains of M. lepraemurium, Hawaii, Kurume No. 42, and Kumamoto, were inoculated to and cultivat-ed in NC-5 medium at 30°C, these strains easily multiplied, whereas there were some differences of growth abilities among the three strains in the case of NC medium.
