When soluble starch was added to NC-5 and ND-5 medium, the growth of M. lepraemurium was not only significantly stimulated, but also maintained until 10 weeks' cultivation at 30°C. Optimal final concentration of soluble starch was 0.2 to 0.4 per cent. In the case of NC-5 medium, many bacterial clumps were observed by the addition effect of soluble starch.
