Abstract
The purpose of this experiment was to obtain a large number of bacilli that could be used for biochemical studies and also for subcultivation experiments. The results obtained are summerized.
1. Yields of M. lepraemurium depended upon the size and form of flask which contained the culture medium.
2. Morphology and other characters of M. lepraemurium were influenced by airspace of culture system.
3. Cultivation of M. lepraemurium in so-called 100ml-flask containing 42ml of complete medium was more efficient than in so-called 50ml-flask from viewpoints of the yieldsand cell-properties.
