Analysis of Mycolic Acids and Various Carbon-Chain-Length's Fatty Acids in Mycobacterium lepraemurium Originated from Rough and Smooth Colonies Grown on Ogawa's Yolk Media
1986 Volume 55 Issue 1 Pages 13-21
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1986 Volume 55 Issue 1 Pages 13-21
Analysis of constitutive fatty acids and assays in viva of fatty acid-synthesizing activity were carried out in respect of two kinds of Mycobacterium lepraemurium, one originated from the rough colonies (5th generation, R-bacilli) and the other from the smooth colonies (80th generation, S-bacilli), both were grown on Ogawa's yolk media.
It was concluded that S-bacilli seemed to have C14-26-fatty-acid-synthesizing abilty more active than R-bacilli, while the former seemed to have C27-58-fatty acid-and mycolic acid-synthesizing abilities less active than the latter.
In spite of a less content of mycolates in the S-bacilli than the R-bacilli, no qualitative difference in mycolates-structure was observed. Using HPLC, Mass-spectrometry as well as H+-NMR-analysis, both bacilli have α-mycolates (containing two cyclopropane-rings), β-mycolates (containing each one of cyclopropane-ring, methy-branch and oxo-group) and dicarboxylic mycolates (containing one cyclopropane-ring). The reliability on chemical identification of mycobacterial species using mycolate-analysis was, therefore, ascertained furthermore.
