Exploration of optimal tissue fixation method for tissue-specific expression of plants

Abstract

Rhizobium is infected with host legume plants and forms nodules, and nitrogen fixing is performed. In recent years, it has been studied that nodule formation involves plant hormones. In particular, the cytokinin signaling system is shown to be involved in the nodulation mechanism. This study aims to explore the optimal tissue fixation method for the study of tissue specific expression of cytokinin signaling genes in legume plants infected Rhizobium.