Hydrogen Production by Fermentation from Food Wastes Containing Starch
2006 Volume 31 Issue 1 Pages 33-37
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2006 Volume 31 Issue 1 Pages 33-37
Fermentative hydrogen production from starch and artificial food waste was studied by using a newly isolated mesophilic bacterium HN001. This bacterium could produce H2 from starch directly. Effect of incubation temperature, cultivation pH and starch concentration were investigated using YNU anaerobic culture. The maximum H2 production rate decreased along with the increase of temperature from 1.2L-H2 L-culture-1 h-1 at 37℃ to 0.3L-H2 L-culture-1 h-1 at 50℃. The production rate was also increased from 0.6L-H2 L-culture-1 h-1 to 1.8L-H2 L-culture-1 h-1 along with the increase of culture pH from 5.5 to 6.5. The maximum H2 production rate did not increase linearly along with the increase of starch concentration but approached nearly fixed rate at 1.5L-H2 L-culture-1 h-1 at over 1.5%-starch. H2 yield decreased linearly along with the increase of starch concentration because of the increased lactic acid production. There were significant differences between cultivation with entrails and without entrails on the maximum H2 production rate, accumulated volume of H2 and metabolites production. This bacterium produced hydrogen approximately 66L at 37℃, pH6.0 from 1kg of wet artificial food waste containing cooked rice and frozen vegetables with entrails of fishes. The hydrogen production was also carried out at repeated batch cultivation.
