Abstract
Enterococcal surface protein (esp) gene in Enterococcus faecium was used as an indicator of humanfecal pollution. A real-time PCR could not detect the esp gene directly in wastewater sample and in any enterococciisolated from sewage influent. Contrastingly, the uidA gene specific to Escherichia coli was detected in wastewatersample, and shown to be useful indicator of fecal pollution. However, it was not detected in low polluted samplessuch as seawater.