The suspension culture medium of rice (cv. Koshihikari) was investigated. Koshihikari's calli proliferated well in DKN liquid medium that contained R2 inorganic salts (KNO_3 and (NH_4)_2SO_4 concentration were diluted to 1/5), B5 vitamins, 5mM aspartic acid, 5mM glutamine and 30g/l sucrose with 1mg/l 2, 4-D.
