Abstract
The genetic diversity and differentiation among 60 individuals of the threatened dragonfly species Libellula angelina from three populations in the Okegayanuma area of Japan was determined using random amplified polymorphic DNA (RAPD) analysis. Twenty polymorphic loci were detected by 19 of the 80 RAPD primers examined, and 12 DNA types were determined (only four types were population specific). The diversity among and within the populations was lower; the mean gene diversity and gene differentiation values were 0.317 and 0.007, respectively. No significant between-population genetic differences were detected in the analysis of molecular variance (AMOVA). Of the genetic divergence, 98.7% was attributable to population divergence and 1.3% to individual differences within a population. Cluster analysis indicated that most individuals from the three populations belonged to the same cluster. Our results provide data that could be used to elucidate genetic diversity in L. angelina populations, using RAPD analysis.
