Abstract
We investigated whether Eustoma grandiflorum (Raf.) Shinn bloomed in vitro using various culture conditions, and whether medium components, photosynthetic photon flux density (PPFD), sucrose concentration, and ventilation start time affected growth and blooming. When modified Hyponex (MH) medium was used, the blooming percentage was highest and plant height was shortest. In addition, when PPFD of over 80 μmol·m−2·s−1 or sucrose concentration of 40 g·L−1 or 60 g·L−1 was used, the height of a blooming plant was observed to be approximately 8 cm. Furthermore, the blooming percentage was highest and plant height was shortest when ventilation was started 4 weeks after seeding. Concurrent with the above observations, we concluded that Eustoma grandiflorum blooms in vitro and the plant height reaches approximately 8 cm when a PPFD of 80 μmol·m−2·s−1 is maintained from seeding to blooming; ventilation start time is 4 weeks after seeding; Murashige-Skoog medium with a sucrose concentration of 30 g·L−1 is used during the 4 weeks, from seeding to first transplantation, and 8 weeks later, from first transplantation to second transplantation, and MH medium with a sucrose concentration of 40 g·L−1 is used from second transplantation to blooming.
