Abstract
The effects of temperature on induction and growth of somatic embryos in cyclamen were investigated. Both the induction of embryogenic callus from explants and formation of somatic embryos from the callus were examined at 20, 25 or 30°C. The calli induced at 20°C formed a markedly smaller number of somatic embryos after subculture on medium without plant growth regulators than calli induced at 25 or 30°C. In vitro germination and growth of somatic embryos were also examined at 15, 20 or 25°C. Obviously, the higher temperature of 25°C inhibited germination and growth. Although the temperature of 20°C was suited for germination, the lower temperature of 15°C was effective for growth of the germinated somatic embryos. These results suggest that the optimal temperature in plant regeneration through somatic embryogenesis in cyclamen differs by stage.
