Abstract
To stabilize the yield of fruit production in Vitis coignetiae Pulliat, which is a dioecious plant that requires pollination for fruiting, we investigated long-term pollen storage using organic solvents. The germination rate which was initially 50.6 %, was reduced to zero after 189 days of storage in low temperature (−20°C) and was also reduced to zero after 117 days of storage in acetone. However, the germination rate of pollen remained 22.8 % after one year of storage in diethyl ether. Artificial pollination of Vitis coignetiae Pulliat using pollen that had been stored for one year in diethyl ether achieved fruiting almost equal to that achieved using fresh pollen. Berry quality did not differ between fruits pollinated by stored pollen and those pollinated by fresh pollen. We conclude that long-term pollen storage over one year with diethyl ether may be a promising for stabilized fruit production in Vitis coignetiae Pulliat.
