2021 Volume 20 Issue 1 Pages 17-27
The development of cultivar identification technology and the protection of breeder’s rights are required for Japanese acid citrus cultivars in conjunction with the increasing domestic production and expanding overseas export of acid citrus fruits. Thirty-three acid citrus cultivars and lines were analyzed using 26 CAPS markers previously applied to the identification of table citrus cultivars. It revealed that all accessions of Yuzu (Citrus junos hort. ex Tanaka), Sudachi (C. sudachi hort. ex Shirai), Yuko (C. yuko hort. ex Tanaka), and Jabara (C. jabara hort. ex Tanaka), which had different phenotypes and local origins, had the same genotypes as their representative samples. In acid citrus cultivars, five CAPS markers detected novel alleles that were not found in table citrus cultivars. Based on the calculation of 25 CAPS markers genotypes except for Tf0001/Msp I by the MinimalMarker program, a minimal marker set comprising 6 CAPS markers can discriminate all 35 citrus cultivars. Parentage analysis of a new triploid cultivar of ‘Awasuzuka’ (C. sudachi (2n = 4x) × C. junos (2n = 2x)) bred in Tokushima Prefecture by the MARCO program indicated that ‘Awasuzuka’ was inherited from either of the parental alleles and showed no discrepancy in the parent-offspring relationship. The establishment of cultivar identification technology for major acid citrus cultivars in the Japanese market reinforces the protection of Japanese brands and breeding rights of superior citrus cultivars.
