2022 Volume 21 Issue 1 Pages 111-122
The development of a cultivar identification system for fresh and processed fruits is required to protect breeder’s rights regarding acid citrus cultivars and for the appropriate management of nursery plants for acid fruit cultivars because their fruits are mainly utilized for cooking and processing. A new set of 10 single nucleotide polymorphism (SNP) markers, which can be used to evaluate the allelic genotype on either locus of 9 citrus chromosomes, has been developed to reinforce the cultivar identification of major acid citrus by improving the previous system for fresh market cultivars. The new set of 10 SNP markers can discriminate the 85 examined citrus cultivars from each other, comprising 10 conventional acid citrus cultivars, 2 acid citrus cultivars released by Tokushima Prefecture and the National Agriculture and Bio-oriented Research Organization (NARO), and 73 fresh market cultivars. The parentage analysis of 10 SNP markers revealed no discrepancy in parent-offspring relationships of 43 combinations in the 85 examined citrus cultivars. They are applicable to SNP genotyping of fresh, and processed fruits such as peel, juice, squeezed vinegar and dry fruit of major acid citrus cultivars. The renewed cultivar identification system is a practical technology that is applicable to the rapid inspection at customs for goods infringing on intellectual property rights and will help protect breeder’s rights pertaining to the registered cultivars.
