Influence of Blooming Stage, Different Parts within the Petal and Dark Treatment on Adventitious Bud Formation in Chrysanthemum Petal Tissue Culture

Abstract

We investigated the influence of the blooming stage, different parts within the petal, and dark treatment on adventitious bud formation in Chrysanthemum morifolium ‘Jimba’ petal tissue culture. In this petal tissue culture, the percentage of adventitious bud formation can be increased by culturing the central part of petals from fully-bloomed flowers after L-Kynurenine treatment in the dark for 10 days. It is suggested that adventitious bud formation involves endogenous plant hormones present in petals. The fact that adventitious buds occurring on the petal after 35 days of tissue culture did not arise from calli suggests that the level of mutation of adventitious buds formed is low.