Abstract
The procedures for tissue culture to establish a mass propagation method for Primula sieboldii E. Morr., were examined. Young expanding leaves were taken from potted plants. After sterilization, leaf segments were cultured on 1/2 MS medium (Murashige·Skoog, 1962) supplemented with 4.44×10-6 M benzyladenine (BA), 5.37×10-7 M α-naphthalene acetic acid (NAA), 3% sucrose and 0.8% agar. There were differences in the potential of leaf segments to induce calli and adventitious shoots among the tested cultivars. To obtain a number of clones, root segments excised from a donor plant in vitro were used as explants. Calli from the root segments were easily induced on MS medium supplemented with BA and NAA. Adventitious shoots were induced easily and then transferred to hormone-free MS medium for 30 days. These adventitious shoots successfully developed into plantlets. The best results for inducing calli in root segment culture were obtained using MS medium with 10-6 M BA incubated under 3,000 1x, a 16 hr photoperiod at 20 or 25°C. Thus, plantlets were easily regenerated from calli. The regenerated plantlets were incubated on vermiculite, and growing plants were then transferred to soil and successfully acclimated.
