Horticultural Research (Japan)
Online ISSN : 1880-3571
Print ISSN : 1347-2658
ISSN-L : 1347-2658
Breeding & Germplasm Resources
Callus Formation and Plant Regeneration from Root Explant of Gladiolus (Gladiolus × grandiflora hort.).
Masakazu KasumiYasumasa TakatsuKazunori SuzukiTakeru GonaiMitsuko NogiTetsuya YamadaToru Manabe
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2005 Volume 4 Issue 1 Pages 7-10

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Abstract

Callus formation, organogenesis and somatic embryogenesis from root section of gladiolus cormels grown in vitro were observed on MS medium containing NAA with and without BAP, respectively. The regeneration rate of adventitious buds showed a high frequency on MS medium containing 5 mg·liter-1 NAA and 5 mg·liter-1 BAP. Regenerated shoots were transferred to MS medium, and subsequently roots developed and corms were formed at the base of the shoots. The corms were released from their dormancy by low-temperature treatment and transplanted into pots, then developed shoots, roots and flowers. The regenerated plants had the same characteristic flower shape and color as the mother plant. Varietal differences were observed in the rate of adventitious buds formation. In conclusion, we showed the most suitable culture condition, for organogenesis and somatic embryogenesis of gladiolus in using root explant.

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© 2005 by Japanese Society for Horticultural Science
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