Abstract
Anthocyanins and flavonols were analyzed by HPLC using inbread lines and varieties of Pansy (Viola × wittrockiana). Each of the six main anthocyanins and flavonols were detected by HPLC. Four anthocyanins were identified by co-chromatography to be Dp3RG, Cy3RG, Dp3pCRG5G (nasunin) and Cy3pCRG5G. One of the flavonols was identified by co-chromatography to be Qu3RG (rutin). Yellow and white lines did not contain anthocyanin. Nasunin, rutin and five flavonols were detected in bluish color. There were differences in the amounts of major flavonols between lines. This result indicated the possibility of anthocyanin-flavonol copigmentation in variations of bluish color. Cyanidin-3-ramnosylglucoside, rutin and five flavonols were detected in reddish flowers. Major flavonols were No. 3, No. 4, and rutin in red lines. Another anthocyanin (Cy3pCRG5G) was detected in reddish-purple line. Differences in anthocyanin influenced the color variation of reddish colors. These findings indicated the importance of HPLC analysis in pansy breeding.
