Comparison between Identification of Anthocyanin by HPLC Analysis with a Photodiode Array Detector and that Using TLC Combined with UV-VIS Spectral Analysis

Abstract

Properties of nine kinds of anthocyanidins and 24 kinds of anthocyanins were compared by thin-layer chromatography (TLC) data and UV-VIS spectrophotometer data with high performance liquid chromatography (HPLC) data including photo diode array (PDA) detector data. The measuring time is about one hour with HPLC analysis though it takes over twelve hours using TLC combined with UV-VIS measurement. Moreover, it is possible to analyze even by a small amount of material using the HPLC analysis, and the efficiency is also high. In addition, absorption spectrum measurement is possible using PDA at the same time. In this study, spectrum data obtained with a PDA detector, which is expected to increase the efficiency of the identification of anthocyanin by HPLC analysis, was presented.