Abstract
To establish of seed propagation methods in Acanthopanax sciadophylloides, GA3 application (200 mg•L−1), warm (20°C) stratification and cold (5°C) stratification procedures were conducted. Seeds in ripening fruits had immature embryos showing globular or heart shapes. GA3 pretreatment accelerated embryo development among plants receiving four-months warm stratification treatment. The effect of cold stratification treatment on embryo development was observed in seeds with GA3 + three-months warm stratification treatment. After four months of warm stratification treatment, however, many seeds decayed during cold stratification. These findings indicated that GA3 treatment prior to warm stratification treatment and following cold stratification treatment promoted embryo development. The most effective method for seed ripening was GA3 pre- and post-treatment plus three months of warm stratification plus four months of cold stratification treatment.
