Abstract
We developed a new method of examining the effects of allelochemical substances exuded from the roots of asparagus (Asparagus officinalis L.) to rhizosphere soil. This rhizosphere soil bioassay method provided results similar to those from the conventional bioassay method in which asparagus germinates in the soil. The new method was more effective than the conventional bioassay method because the soil samples could be tested using a smaller volume. This method could also evaluate flowable activated carbon as one of the materials to absorb allelochemical substances at a laboratory level. An appropriate orientation of cultivation is expected to become possible using the rhizosphere soil assay method developed in this study to examine obstructions to continuous cropping that may be related to allelopathy.