Abstract
For HPLC analysis of soluble carbohydrate concentration in petals, a simple and rapid extraction method using a centrifugal filter device was developed. Rose ‘Sonia’ petals were placed in a centrifugal filter device, frozen in liquid nitrogen and centrifuged. The resulting leached fluid was used as a soluble carbohydrate sample for HPLC analysis. The amount of leached fluid increased with the increasing duration of centrifugation, but reached a plateau after centrifugation for 30 min. When soluble carbohydrate composition of fluid obtained from this method was compared with that from a conventional extraction method using hot ethanol, there was no significant difference between the two methods. When leached fluid was held at room temperature, its sucrose concentration decreased with time, but this decrease was suppressed when the fluid was stored at 4°C. This suggests that soluble carbohydrate samples prepared by this method should be stored at a low temperature and analyzed as soon as possible. Soluble carbohydrate samples were extracted from rose ‘Madeleine’, ‘New Bridal’, ‘Rote Rose’, ‘Saturn’, carnations, Eustoma and Oxypetalum petals using this method and the compositions of these samples were compared with those obtained by the conventional method. There were no marked differences between the two methods. These results suggested that the extraction method using a centrifugal filter device is a simple and rapid method of determining soluble carbohydrate concentrations in the petals of various flowers.
