Abstract
Using 7 pairs of simple sequence repeat (SSR) primers developed for Japanese chestnut, we identified the SSR genotypes of 7 chestnut cultivars that originated from the Korean peninsula and compared them with those of 17 Chinese chestnut cultivars, 32 Japanese chestnut cultivars, and an interspecific hybrid obtained by crossing the above species. The 7 SSR loci of all cultivars were successfully genotyped, except KT006a for which no amplification products were obtained from 3 cultivars of Chinese chestnut. Among the 36 alleles observed in the 17 Chinese cultivars, 26 were shown to be species-specific alleles, while 37 of the 47 alleles observed in the 32 Japanese cultivars were shown to be specific to Japanese chestnut. The SSR genotypes of the Korean cultivars were also identified and comprised 2-9 alleles per locus (average: 5.14 alleles). By comparing the results to the species-specific alleles, we considered that ‘Myeonsan 9’, ‘Myeonsan 13’, ‘Myeonsan 60’, ‘Inhungwanglyul’, and ‘Han 6’ are Japanese chestnut cultivars; ‘Hamjong 3 ho’ is a Chinese chestnut cultivar; and ‘Ooshiro’ is an interspecific hybrid. A synonymous relationship or a bud mutation was estimated between ‘Han 6’ and ‘Tanzawa’, and between ‘Ooshiro’ and ‘Riheiguri’.
