Abstract
To clarify the origin of local radish variety ‘Sabaka’ in Kyoto prefecture, the presence and variations of mitochondrial male-sterile gene (orf138) and the fertility restorer gene, orf687, were investigated. Five plants of ‘Sabaka’ and 97 Japanese wild radish plants collected in Maizuru City, Kyoto prefecture, were used. After isolating total DNA from a young leaf of each plant, PCR and PCR-RFLP analyses were conducted for orf138 and orf687 gene with DNA as the template. All five plants of ‘Sabaka’ possessed a unique orf138 sequence (Type E) that has not been observed at all in cultivated radishes, and ‘Yuan hong’ type RFLP for orf687. In wild radishes, 25 plants had orf138 and three of them were classified into Type E. Whereas, 14 wild radish plants possessed ‘Yuan hong’ type orf687 similarly to ‘Sabaka’, among which two plants were Type E for orf138. These findings indicate that ‘Sabaka’ originated from the domestication of Japanese wild radish having both male-sterile cytoplasm with orf138 and the fertility restorer gene, orf687.
