Abstract
Many methods of bile analysis for various species of man and mammals have been reported, but they are difficult to employ for minute quantities due to the inherent inaccuracy of the processes. In this paper the analysis of the bile acids of Elaphe quadrivirgata (Boie) by thin layer chromatography is reported. To purify the sample of bile acids, extraction with organic solvents and hydrolysis were used. The sample was separated into 12 fractions on the stationary phase of silica GH by two-demensional development. Taurocholic acid was clearly identified, but the identity of the other spots remained unclear.
