2001 Volume 24 Issue 4 Pages 411-419
The aim of this study was to evaluate the possible role of prostacyclin (PGI2) in the onset and development of hypertension and chronic renal failure in 5/6-nephrectomized rats (5/6 NX). We measured the systolic blood pressure, 24-h urinary excretion levels of 6-keto-PGF1α, which was a stable metabolite of PGI2, and levels of PGI2 synthase (PCS) mRNA in the kidneys. Immunostaining for PCS in the kidneys was also evaluated. Systolic blood pressure was higher in 5/6 NX than in sham-operated rats. The 24-h urinary excretion levels of 6-keto-PGF1α in 5/6 NX at 1 week postsurgery were lower than in sham-operated rats. In renal morphology, tubulointerstitial injury was observed at 2 weeks postsurgery, and glomerulosclerosis at 4 weeks. Levels of PCS mRNA in 5/6 NX decreased significantly at 1 and 2 weeks postsurgery compared with those in sham-operated rats, but at 8 weeks these levels showed a tendency to increase. Immunostaining for PCS was positive in a subset of the cortical thick ascending limb of Henle’s loop cells, including macula densa in both groups. Moreover, in 5/6 NX at 8 weeks postsurgery, mesangial cells also stained positive for PCS. In conclusion, our findings suggest that PCS might play an important role in mitigating glomerular hemodynamic changes associated with reduction of renal mass. (Hypertens Res 2001; 24: 411-419)
