Abstract
To understand the relationship between neuronal-network functions and single-neuron activity, construction of artificial neuronal network is one of the promising approaches. Cell patterning is a useful technique to get single-neuron-based networks in vitro. Here in this work, we propose a simple method to get simple neuronal networks, based on neurite-outgrowth guidance. Our method, referred to as “micropipette drawing” is a quite simple photomask-free technique. Growth-guiding patterns are drawn with a micropipette containing cell-adhesive solution on non-adhesive substrates. Guiding structures of approximately 10 μm width were successfully drawn and rat hippocampal neurons were cultured on the patterns. The patterned neuronal networks could be maintained for more than a week.
