Device Fabrication for Creating Multi-scale Miniature Neuronal Networks and Spatiotemporal Pattern Analysis of Spontaneous Electrical Activities

Abstract

To investigate the dynamics of neuronal networks, the device which is capable of multipoint simultaneous measurement and multi-scale miniature neuronal networks construction was fabricated. The result of culturing rat embryo hippocampal neurons showed that the device is biocompatible and can be successfully used in long-term culture. Analysis of the electrical data obtained with the device suggested that, in miniature neuronal networks consisted of about 2000 neurons, there were phenomena of spatiotemporal activity patterns of neurons which were considered to be very important to the process of storing and transforming information of a brain.