Effect of Magnetic Nanoparticle Internalization on Cell Density in Skeletal Muscle Tissue

Abstract

The engineering of skeletal muscle tissue has gained considerable attention in recent years because of its clinical applications, which include muscle replacement and treatment of muscle-related disease. Magnetic nanoparticle internalization by skeletal muscle cells has been proposed as a means of improving cell density. In this study, by embedding magnetic nanoparticles in mouse C2C12 myoblast cells to engineer a muscle tissue sheet, we evaluated the cellular uptake of magnetic nanoparticles and their effects on cell proliferation and differentiation. Our results show a tendency for decreased muscle fiber thickness in C2C12 cells with internalized magnetite nanoparticles. However, no change was found in the number of cells or differentiation.