Changes in Cardiac Myofibrillar ATPase Activity during Development of Hyperthyroidism in the Rabbit

Abstract

This study examined changes in cardiac myofibrillar ATPase activity during the development of hyperthyroidism and the relationships between changes in myofibrillar ATPase activity and hemodynamic parameters in rabbits. Calcium-stimulated, magnesium-dependent ATPase (Ca-ATPase) activity of cardiac myofibrils exhibited about a 30% increase after the first week of treatment with excess desiccated thyroid tissue powder (thyroid-treatment) and did not show further increase during thyroid-treatment. Development of cardiac hypertrophy and increases in systolic and diastolic blood pressure were dependent upon prolonged periods of thyroid-treatment lasting from 3 days to 4 weeks, whereas the maximal increase (about 55%) in heart rate was seen after the first week of thyroid-treatment. The elevated level lasted throughout subsequent thyroid-treatment. Lineweaver-Burk plots showed an increase in Km and Vmax values for Ca-ATPase activity of my-ofibrillar preparation from the thyrotoxic hearts of treated animals. Increased Ca-ATPase activity was correlated with thyroid-induced changes in heart rate. Propranolol partially reduced the thyroidinduced increase in Ca-ATPase activity and heart rate, suggesting participation of beta-adrenoceptors in the thyroid-induced increases of Ca-ATPase activity and heart rate.