A Method for the Determination of Plasma Renin Activity in Small Samples

Masashi IMAI; Hirofumi SOKABE

doi:10.1536/ihj.8.607

Masashi IMAI, Hirofumi SOKABE

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JOURNAL FREE ACCESS

1967 Volume 8 Issue 6 Pages 607-619

DOI https://doi.org/10.1536/ihj.8.607

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Published: 1967 Received: April 12, 1967 Available on J-STAGE: December 09, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: December 09, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : (1) Effects of Dowex 50W-X2 resin, diisopropyl fluorophosphate, and incubation at pH5.5 were compared for inhibiting angiotensinase activity in plasma with 0.93×10^-2M EDTA. We found that Dowex 50W-X2 is reliable.
(2) The method of Boucher et al. for determination of plasma renin activity using the Dowex resin was re-examined. We modified the procedures for the determination in small amount of samples.
(3) We measured angiotensinogen content of the plasma simultaneously, and expressed renin activity by the reaction constant of first order kinetics (K), since a linear correlation was found between amount of renin and the reaction constant.
(4) Evidences were presented that the cource of plasma renin-angiotensinogen reaction can be also approximated by the first order kinetics in our system.
(5) Recovery of angiotensin throughout the procedures was 74±12 (standard deviation)%. The results were sufficiently reproducible.
Right : In order to establish a reliable method for the determination of plasma renin activity in small samples, we have compared the methods reported. The method of Boucher et al. was found to be reliable, and modified in the laboratory. Expression of renin activity by the first order reaction constant was emphasized.

Date of correction: December 09, 2008 Reason for correction: - Correction: AUTHOR Details: Wrong : Masashi IMAI¹⁾²⁾, Hirofumi SOKABE¹⁾²⁾
Right : Masashi IMAI¹⁾, Hirofumi SOKABE²⁾

Date of correction: December 09, 2008 Reason for correction: - Correction: AFFILIATION Details: Wrong :
1) Department of Pediatrics, Faculty of Medicine, University of Tokyo
2) Department of Pharmacology, Toho University School of Medicine

Right :
1) the Department of Pediatrics, Faculty of Medicine, University of Tokyo
2) the Department of Pharmacology, Toho University School of Medicine

Date of correction: December 09, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 1. Davis, J.O.: Physiologist 5:65, 1962.
2. Peart, W.S.: Pharmacol. Rev. 17:143, 1965.
3. Boucher, R., Veyrat, R., De Champlain, J., and Genest, J.: Canad. Med. Ass. J. 90: 194, 1964.
4. Helmer, O.M. and Judson, W.E.: Circulation 27:1050, 1963.
5. Plentl, A.T. and Page, I.H.: J. Exp. Med. 78:367, 1943.
6. Bumpus, F.M.: Report at the International Symposium on Renal Hypertension, Cleveland, 1966.
7. Kawabe, K. and Sokabe, H.: Jap. Heart J. 6:51, 1965.
8. Harigaya, S., Sokabe, H., and Sakai, F.: Jap. Heart J. 7:66, 1966.
9. Yoshinaga, K., Aida, M., Maebashi, M., Sato, T., Abe, K., and Miwa, I.: Tohoku J. Exp. Med. 80:32, 1963.
10. Brown, J.J., Davies, D.L., Lever, A.F., Robertson, J.I.S., and Tree, M.: Biochem. J. 93:594, 1964.
11. Pickens, P.T., Bumpus, F.M., Lloyd, A.M., Smeby, R.R., and Page, I.H.: Circulat. Res. 17:438, 1965.
12. Warzynski, R., Demirjian, Y., and Hoobler, S.: Canad. Med. Ass. J. 90:225, 1964.
13. De Vito, E. and Fasciolo, J.C.: Acta Physiol. Latinoam. 15:129, 1965.
14. Gould, A.B., Skeggs, L.T., and Kahn, J.R.: Lab. Invest. 15:1802, 1966.
15. Yamaura, Y.: Shin Ikagaku (4th ed.), Nanzando, Tokyo, p. 296, 1964 (in Japanese).
16. Khairallah, P.A., Bumpus, F.M., Page, I.H., and Smeby, R.R.: Science 140:672, 1963.
17. Helmer, O.M.: Canad. Med. Ass. J. 90:221, 1964.
18. Veyrat, R., De Champlain, J., Boucher, R., and Genest, J.: Canad. Med. Ass. J.: 90:215, 1964.
19. Imai, M., Igarashi, Y., and Sokabe, H.: (in preparation).
20. Haas, E. and Goldblatt, H.: Circulat. Res. 20:45, 1967.

Right : 1. Davis, J.O.: Physiologist 5: 65, 1962.
2. Peart, W. S.: Pharmacol. Rev. 17: 143, 1965.
3. Boucher, R., Veyrat, R., De Champlain, J., and Genest, J.: Canad. Med. Ass. J. 90: 194, 1964.
4. Helmer, O. M. and Judson, W. E.: Circulation 27: 1050, 1963.
5. Plentl, A. T. and Page, I. H.: J. Exp. Med. 78: 367, 1943.
6. Bumpus, F. M.: Report at the International Symposium on Renal Hypertension, Cleveland, 1966.
7. Kawabe, K. and Sokabe, H.: Jap. Heart J. 6: 51, 1965.
8. Harigaya, S., Sokabe, H., and Sakai, F.: Jap. Heart J. 7: 66, 1966.
9. Yoshinaga, K., Aida, M., Maebashi, M., Sato, T., Abe, K., and Miwa, I.: Tohoku J. Exp. Med. 80: 32, 1963.
10. Brown, J. J., Davies, D. L., Lever, A. F., Robertson, J. I. S., and Tree, M.: Biochem. J. 93 594, 1964.
11. Pickens, P. T., Bumpus, F. M., Lloyd, A. M., Smeby, R. R., and Page, I. H.: Circulat. Res. 17: 438, 1965.
12. Warzynski, R., Demirjian, Y., and Hoobler, S.: Canad. Med. Ass. J. 90: 225, 1964.
13. De Vito, E. and Fasciolo, J. C.: Acta Physiol. Latinoam. 15: 129, 1965.
14. Gould, A. B., Skeggs, L. T., and Kahn, J. R.: Lab. Invest. 15: 1802, 1966.
15. Yamaura, Y.: Shin Ikagaku (4th ed.), Nanzando, Tokyo, p. 296, 1964 (in Japanese).
16. Khairallah, P. A., Bumpus, F. M., Page, I. H., and Smeby, R. R.: Science 140: 672, 1963.
17. Helmer, O. M.: Canad. Med. Ass. J. 90: 221, 1964.
18. Veyrat, R., De Champlain, J., Boucher, R., and Genest, J.: Canad. Med. Ass. J.: 90: 215, 1964.
19. Imai, M., Igarashi, Y., and Sokabe, H.: (in preparation).
20. Haas, E. and Goldblatt, H.: Circulat. Res. 20: 45, 1967.

Date of correction: December 09, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

In order to establish a reliable method for the determination of plasma renin activity in small samples, we have compared the methods reported. The method of Boucher et al. was found to be reliable, and modified in the laboratory. Expression of renin activity by the first order reaction constant was emphasized.

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