Analysis of the Association between Mesiodens and SNP Genotyping

Abstract

　A mesiodens is the most common supernumerary tooth present in the maxillary incisor area. There have been many reports regarding the prevalence and treatment for supernumerary teeth; however, the etiology of the condition is still unknown. This study aimed to identify genes associated with susceptibility to mesiodens formation. The study population comprised 24 patients with mesiodens and 24 controls. Genomic DNA was obtained from the soft tissue of extracted supernumerary teeth or saliva samples. Genotypes and allele frequencies of single nucleotide polymorphisms (SNPs) in genes associated with the initiation of odontogenesis, including TP63, PITX2, LEF1, BMP2, BMP4, FGF9, FGF20, WNT10A, WNT10B, EDA, EDAR, MSX1, MSX2, PAX9, LHX6, and RUNX2 were compared between mesiodens group and controls using chisquared tests. In addition, SNPs in genes associated with supernumerary incisor formation in rodents, including SOSTDC1, LRP4, APC, PAX6, and CEBPB were tested. No positive polymorphisms were found in the tested SNPs between the two groups. These results suggest that these SNPs might not be candidates of genetic marker for mesiodens formation.