Abstract
Possessing sufficient antimicrobial properties in saliva may prevent the excessive growth of fungus and intraoral indigenous bacteria to prevent the oral mucosal disease and pneumonia. The purpose of this study was to clarify the intraday and day-to-day fluctuations of three antimicrobial properties: beta-defensin, histatin, and IgA, which inhibit the growth of C. albicans and intraoral bacteria. Twenty healthy students or workers(10
males and 10 females; 25.7±1.95 years) at Nihon University School of Dentistry at Matsudo were recruited. Resting saliva was collected daily from 10 to 11 AM, and from 15
to 16 PM, for seven days. By using ELISA, individual concentrations(ng/mL) of the antimicrobial properties were examined. The two-way ANOVA analyzed the effect of dayto-day and intraday fluctuations. No significant day-to-day fluctuations in beta-defensin 3(p =0.13), histatin 5(p=0.22), and IgA(p=0.36) were detected. Also, no significant intraday fluctuations of beta-defensin 3(p=0.58) and histatin 5(p=0.70) were detected. However,IgA showed significant low concentration in the morning(p=0.002) than in the afternoon.
The results indicate that the saliva sampling in the morning, rather than the afternoon, is optimal for future studies of host immune factors: beta-defensin, histatin, and IgA.
