MicroRNA Profiling in Exosomes from Conditioned Medium of Tumor Necrosis Factor - α or Interleukin - 1β Stimulated Human Gingival Fibroblasts

Abstract

Exosomes, also known as extracellular vesicles, are produced from variety of sources, including body fluids and cultured cells. Etiology of periodontitis is complex, and microRNAs（miRNAs）in the exosomes may play an important role for medical condition. The purpose of this study was to analyze the expression profiles of miRNAs in the exosomes from human gingival fibroblasts（HGF）with or without stimulation by inflammatory cytokines. Total RNAs were isolated from exosomes. Expression profiles of miRNAs was studied by miRNA microarrays, GeneSpring GX, Ingenuity Pathways Analysis（IPA）and TargetScan databases. MiRNA expression levels were confirmed by real-time polymerase chain reaction. Exosomes from the conditioned medium of HGF stimulated by tumor necrosis factor-α（TNF-α）or interleukin - 1β（IL-1β）contained 18 or 23 overexpressed miRNAs. Three most overexpressed miRNAs in the exosomes were hsa-miR-24-3p, hsa-miR-23a-3p and hsa-let-7c-5p for TNF-α or hsa-miR-24-3p, hsa-miR -3665-5p and hsa-miR-6089-3p for IL-1β stimulation. In IPA analysis, hsa - miR-3665-5p was involved in hereditary disorder, neurological disease, organismal injury and abnormalities, hsa-miR-149-3p was associated with cardiac dilation and enlargement, cardiovascular disease and hsa-miR-455-3p was related to cellular development, skeletal, muscular and cardiovascular system development and function. These findings suggest that miRNAs in the exosomes from HGF stimulated by TNF-α or IL-1β might be associated with skeletal, muscular and cardiovascular development and function and inflammation of periodontium.