Abstract
In these years, for medical analysis, many 3D microscopes have been developed. In the existing 3D microscopes, confocal laser scanning microscope (CLSM) is used mostly. 3D shape of biological tissues can be measured with high-resolution. However scanning, which is necessary during measurement, takes time consuming. So it is difficult to measurement living biological tissues, such as the red blood cells. On the other hand, measurement accuracy of the CLSM is about 1[µm], so 3D measurement using the CLSM is not high accuracy. To resolve problems in the CLSM, we propose a 3D measurement method using single-shot phase-shift digital holography. Using the proposed method, we also developed a 3D microscopy for 3D measurement of red blood cells with 5.5[nm] measurement accuracy and 80[ms] measurement time. This paper describes the detail of the proposed 3D measurement method and the 3D microscopy.
