Abstract
The author conducted experiments in order to find the staining method, to be manipulated simply, to be conducted rapidly and to be suitable for morphological investigation and discrimination of species Protozoae, and obtained results that 10% of formalin solution for fixing and dilute Giemsa liquid, 1 drop per 2 ml of phosphate buffer solution or 2 drops per 3 ml, were respectively the best ones, which will be distinguishable to demonstrate flogella, nucleus and other detailed structures of trichomonas elongata.
The technic shall be proceeded as follows;
The material and the fixing solution of 1/2 to 113 in quantity are mixed on slide glass, layed aside for 10 to 20 minutes, and spread thoroughly over the slide glass. When the circumference of the smear becomes dry, and the slide is dripped in the staining solution and kept in it for 6 hours at 37°C. Further process shall be as usual.
