Abstract
The present paper deals with the study of trichomonas elongata under fluorescent microscopic observation, dyed with acridin-orange.
Acridin-orange did not gave strong fluorescence of flagella, however, it showed many new foundings which could not be shown by auramin. Acridin-orange gave rose fluorescence in various granula and flesh green fluorescence in neuclea. Consequently, by the varied contrast color of granula and nuclear, living organism could be differentiated from dead one. Wet preparation which had been treated with 1: 3, 000-7, 400 dilution of acridin-orange is useful for investigating the vitality of trichomonas elongata.
In the further investigation as to influence of acridin-orange to the growth and proliferation of the protozoa, it was found that trichomonas elongata can grow well in the concentration of 15, 000 fold or more high dilution of acridin-orange, and that the protozoa that had been once treated with the pigment will exert fluorescence in fresh medium of transplantation, even in the 3rd medium.
